Patient-derived tumor tissue lines

Transplantable cancer tissue xenografts (living tumor tissue lines) are based on grafting patients’ biopsy or excised tumor tissue into the subrenal capsule graft site (sub-renal capsule xenografting). The high vascularity of this site, compared to subcutaneous and orthotopic sites, allows more adequate supply of nutrients to the graft important for maintaining tumor heterogeneity. Thus, the cancer tissue xenografts established in the Living Tumor Laboratory have the following advantages:

• High take rate. We have consistently achieved high tumor take rates (>95%) for a variety of low and higher grade malignancies.
• Retention of major characteristics of the original tumors. The tumor tissue lines retain major genotypic and phenotypic characteristics (histopathology, heterogeneous cell population, target expression and target dependence) of the patient’s original tumor, including drug sensitivity/resistance. Early samples are stored frozen in liquid nitrogen.
• Novel model of metastasis. Many of our transplantable living tumor tissue lines exhibit predictable macroscopic (high-volume) metastases.
• High reproducibility. Large amounts of tumor tissue can be grown readily.

Overview of Xenograft Models in the Living Tumor Laboratory

The Living Tumor Laboratory has established more than 200 transplantable human cancer tissue lines in SCID mice directly derived from a wide range of primary tumors from patients.

Overview of human cancer tissue lines at the Living Tumor Laboratory

Tumor origin

No. of lines

Histopathology type

Prostate

45

Adenocarcinoma

Neuroendocrine carcinoma

CRPC

Lung

59

Squamous cell carcinoma, Adenocarcinoma
Large cell carcinoma
Small cell carcinoma
Other

Ovary

51

Serous carcinoma
Mucinous carcinoma
Endometriod carcinoma
Clear cell carcinoma
Undifferentiated carcinoma

Dysgerminoma

Breast

5

Invasive ductal carcinoma

Uterus

9

Sarcoma

Leiomyosarcoma

Serous carcinoma

Cervical carcinoma

Pancreas

5

Adenocarcinoma

Kidney and urinary bladder

13

Clear cell renal cell carcinoma

Urothelia (transitional cell) carcinoma

Lymphoid tissue

9

Diffuse large B cell lymphoma  

Colon

2

Malignant gastrointestinal stromal tumor

Skin

7

Malignant melanoma

Bone

1

Osteosarcoma

Total

205

 

 *Note: An established sub-renal capsule tumor tissue line may also be transplanted subcutaneously or orthotopically.